Unknown

Dataset Information

0

Axolotl Nanog activity in mouse embryonic stem cells demonstrates that ground state pluripotency is conserved from urodele amphibians to mammals.

ABSTRACT: Cells in the pluripotent ground state can give rise to somatic cells and germ cells, and the acquisition of pluripotency is dependent on the expression of Nanog. Pluripotency is conserved in the primitive ectoderm of embryos from mammals and urodele amphibians, and here we report the isolation of a Nanog ortholog from axolotls (axNanog). axNanog does not contain a tryptophan repeat domain and is expressed as a monomer in the axolotl animal cap. The monomeric form is sufficient to regulate pluripotency in mouse embryonic stem cells, but axNanog dimers are required to rescue LIF-independent self-renewal. Our results show that protein interactions mediated by Nanog dimerization promote proliferation. More importantly, they demonstrate that the mechanisms governing pluripotency are conserved from urodele amphibians to mammals.

SUBMITTER: Dixon JE 

PROVIDER: S-EPMC2926951 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Axolotl Nanog activity in mouse embryonic stem cells demonstrates that ground state pluripotency is conserved from urodele amphibians to mammals.

Dixon James E JE   Allegrucci Cinzia C   Redwood Catherine C   Kump Kevin K   Bian Yuhong Y   Chatfield Jodie J   Chen Yi-Hsien YH   Sottile Virginie V   Voss S Randal SR   Alberio Ramiro R   Johnson Andrew D AD  

Development (Cambridge, England) 20100901 18

Cells in the pluripotent ground state can give rise to somatic cells and germ cells, and the acquisition of pluripotency is dependent on the expression of Nanog. Pluripotency is conserved in the primitive ectoderm of embryos from mammals and urodele amphibians, and here we report the isolation of a Nanog ortholog from axolotls (axNanog). axNanog does not contain a tryptophan repeat domain and is expressed as a monomer in the axolotl animal cap. The monomeric form is sufficient to regulate plurip  ...[more]

Similar Datasets

Unknown Conserved long noncoding RNAs transcriptionally regulated by Oct4 and Nanog modulate pluripotency in mouse embryonic stem cells.
| S-EPMC2811662 | biostudies-literature
Unknown Functional diversity of voltage-sensing phosphatases in two urodele amphibians.
| S-EPMC4187576 | biostudies-literature
Unknown Honey bee Royalactin unlocks conserved pluripotency pathway in mammals.
| S-EPMC6277453 | biostudies-literature
Unknown Courtship Pheromone Use in a Model Urodele, the Mexican Axolotl (Ambystoma mexicanum).
| S-EPMC4740750 | biostudies-literature
Unknown Tracking the embryonic stem cell transition from ground state pluripotency.
| S-EPMC5399622 | biostudies-literature
Unknown Super-enhancers conserved within placental mammals maintain stem cell pluripotency.
| S-EPMC9546576 | biostudies-literature
Unknown The pluripotency factor Nanog regulates pericentromeric heterochromatin organization in mouse embryonic stem cells.
| S-EPMC4863740 | biostudies-literature
Unknown NODAL Secures Pluripotency upon Embryonic Stem Cell Progression from the Ground State.
| S-EPMC5511111 | biostudies-literature
Unknown Embryonic stem cells become mechanoresponsive upon exit from ground state of pluripotency.
| S-EPMC6367133 | biostudies-literature
Unknown The pluripotency transcription factor Nanog represses glutathione reductase gene expression in mouse embryonic stem cells.
| S-EPMC6604252 | biostudies-literature