Ontology highlight
ABSTRACT:
SUBMITTER: Wu P
PROVIDER: S-EPMC2929323 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Wu Peng P van Overbeek Megan M Rooney Sean S de Lange Titia T
Molecular cell 20100708 4
Mammalian telomeres contain a single-stranded 3' overhang that is thought to mediate telomere protection. Here we identify the TRF2-interacting factor Apollo as a nuclease that contributes to the generation/maintenance of this overhang. The function of mouse Apollo was determined using Cre-mediated gene deletion, complementation with Apollo mutants, and the TRF2-F120A mutant that cannot bind Apollo. Cells lacking Apollo activated the ATM kinase at their telomeres in S phase and showed leading-en ...[more]