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Hoechst-IR: an imaging agent that detects necrotic tissue in vivo by binding extracellular DNA.

ABSTRACT: Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction and lipopolysaccharide/d-galactosamine (LPS-GalN) induced sepsis.

SUBMITTER: Dasari M

PROVIDER: S-EPMC2929653 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature

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Hoechst-IR: an imaging agent that detects necrotic tissue in vivo by binding extracellular DNA.

Dasari Madhuri M Lee Sungmun S Sy Jay J Kim Dongin D Lee Seungjun S Brown Milton M Davis Michael M Murthy Niren N

Organic letters 20100801 15

Cell necrosis is central to the progression of numerous diseases, and imaging agents that can detect necrotic tissue have great clinical potential. We demonstrate here that a small molecule, termed Hoechst-IR, composed of the DNA binding dye Hoechst and the near-infrared dye IR-786, can image necrotic tissue in vivo via fluorescence imaging. Hoechst-IR detects necrosis by binding extracellular DNA released from necrotic cells and was able to image necrosis generated from a myocardial infarction ...[more]

PMID: 20597468

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