Ontology highlight
ABSTRACT:
SUBMITTER: Cho IM
PROVIDER: S-EPMC2930468 | biostudies-literature | 2010 Aug
REPOSITORIES: biostudies-literature
Cho I-Ming IM Lai Lien B LB Susanti Dwi D Mukhopadhyay Biswarup B Gopalan Venkat V
Proceedings of the National Academy of Sciences of the United States of America 20100730 33
To the mounting evidence of nonribosomal functions for ribosomal proteins, we now add L7Ae as a subunit of archaeal RNase P, a ribonucleoprotein (RNP) that catalyzes 5'-maturation of precursor tRNAs (pre-tRNAs). We first demonstrate that L7Ae coelutes with partially purified Methanococcus maripaludis (Mma) RNase P activity. After establishing in vitro reconstitution of the single RNA with four previously known protein subunits (POP5, RPP21, RPP29, and RPP30), we show that addition of L7Ae to thi ...[more]