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Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells.


ABSTRACT: Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated 'gene silencing' of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.

SUBMITTER: Wang W 

PROVIDER: S-EPMC2932882 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells.

Wang Wei W   Wang Li L   Li Xin-xiu XX   Chen Xia X   Zhang Hai-yan HY   He Yu Y   Wang Jing-jing JJ   Zhao Yong-yan YY   Zhang Bao-le BL   Xu Yin-xue YX  

Journal of Zhejiang University. Science. B 20100901 9


Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated 'gene s  ...[more]

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