Ontology highlight
ABSTRACT:
SUBMITTER: Fordyce PM
PROVIDER: S-EPMC2937095 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Fordyce Polly M PM Gerber Doron D Tran Danh D Zheng Jiashun J Li Hao H DeRisi Joseph L JL Quake Stephen R SR
Nature biotechnology 20100829 9
Gene expression is regulated in part by protein transcription factors that bind target regulatory DNA sequences. Predicting DNA binding sites and affinities from transcription factor sequence or structure is difficult; therefore, experimental data are required to link transcription factors to target sequences. We present a microfluidics-based approach for de novo discovery and quantitative biophysical characterization of DNA target sequences. We validated our technique by measuring sequence pref ...[more]