Ontology highlight
ABSTRACT:
SUBMITTER: Chen D
PROVIDER: S-EPMC5024299 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Chen Dana D Orenstein Yaron Y Golodnitsky Rada R Pellach Michal M Avrahami Dorit D Wachtel Chaim C Ovadia-Shochat Avital A Shir-Shapira Hila H Kedmi Adi A Juven-Gershon Tamar T Shamir Ron R Gerber Doron D
Scientific reports 20160915
Transcription factors (TFs) alter gene expression in response to changes in the environment through sequence-specific interactions with the DNA. These interactions are best portrayed as a landscape of TF binding affinities. Current methods to study sequence-specific binding preferences suffer from limited dynamic range, sequence bias, lack of specificity and limited throughput. We have developed a microfluidic-based device for SELEX Affinity Landscape MAPping (SELMAP) of TF binding, which allows ...[more]