Ontology highlight
ABSTRACT:
SUBMITTER: Kuhn-Holsken E
PROVIDER: S-EPMC2938196 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Kühn-Hölsken Eva E Lenz Christof C Dickmanns Achim A Hsiao He-Hsuan HH Richter Florian M FM Kastner Berthold B Ficner Ralf R Urlaub Henning H
Nucleic acids research 20100426 16
Mass spectrometry allows the elucidation of molecular details of the interaction domains of the individual components in macromolecular complexes subsequent to cross-linking of the individual components. Here, we applied chemical and UV cross-linking combined with tandem mass-spectrometric analysis to identify contact sites of the nuclear import adaptor snurportin 1 to the small ribonucleoprotein particle U1 snRNP in addition to the known interaction of m(3)G cap and snurportin 1. We were able t ...[more]