Ontology highlight
ABSTRACT:
SUBMITTER: Bruckmann C
PROVIDER: S-EPMC7545097 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Bruckmann Chiara C Tamburri Simone S De Lorenzi Valentina V Doti Nunzianna N Monti Alessandra A Mathiasen Lisa L Cattaneo Angela A Ruvo Menotti M Bachi Angela A Blasi Francesco F Blasi Francesco F
Scientific reports 20201008 1
Both onco-suppressor PREP1 and the oncogene MEIS1 bind to PBX1. This interaction stabilizes the two proteins and allows their translocation into the nucleus and thus their transcriptional activity. Here, we have combined cross-linking mass-spectrometry and systematic mutagenesis to detail the binding geometry of the PBX1-PREP1 (and PBX1-MEIS1) complexes, under native in vivo conditions. The data confirm the existence of two distinct interaction sites within the PBC domain of PBX1 and unravel dif ...[more]