Ontology highlight
ABSTRACT:
SUBMITTER: Villalobos V
PROVIDER: S-EPMC2943495 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Villalobos Victor V Naik Snehal S Bruinsma Monique M Dothager Robin S RS Pan Mei-Hsiu MH Samrakandi Mustapha M Moss Britney B Elhammali Adnan A Piwnica-Worms David D
Chemistry & biology 20100901 9
Understanding the functional complexity of protein interactions requires mapping biomolecular complexes within the cellular environment over biologically relevant time scales. Herein, we describe a set of reversible multicolored heteroprotein complementation fragments based on various firefly and click beetle luciferases that utilize the same substrate, D-luciferin. Luciferase heteroprotein fragment complementation systems enabled dual-color quantification of two discrete pairs of interacting pr ...[more]