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Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.

ABSTRACT: Drosophila melanogaster is a model organism rich in genetic tools to manipulate and identify neural circuits involved in specific behaviors. Here we present a technique for two-photon calcium imaging in the central brain of head-fixed Drosophila walking on an air-supported ball. The ball's motion is tracked at high resolution and can be treated as a proxy for the fly's own movements. We used the genetically encoded calcium sensor, GCaMP3.0, to record from important elements of the motion-processing pathway, the horizontal-system lobula plate tangential cells (LPTCs) in the fly optic lobe. We presented motion stimuli to the tethered fly and found that calcium transients in horizontal-system neurons correlated with robust optomotor behavior during walking. Our technique allows both behavior and physiology in identified neurons to be monitored in a genetic model organism with an extensive repertoire of walking behaviors.

SUBMITTER: Seelig JD

PROVIDER: S-EPMC2945246 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior.

Seelig Johannes D JD Chiappe M Eugenia ME Lott Gus K GK Dutta Anirban A Osborne Jason E JE Reiser Michael B MB Jayaraman Vivek V

Nature methods 20100606 7

Drosophila melanogaster is a model organism rich in genetic tools to manipulate and identify neural circuits involved in specific behaviors. Here we present a technique for two-photon calcium imaging in the central brain of head-fixed Drosophila walking on an air-supported ball. The ball's motion is tracked at high resolution and can be treated as a proxy for the fly's own movements. We used the genetically encoded calcium sensor, GCaMP3.0, to record from important elements of the motion-process ...[more]

PMID: 20526346

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