Unknown

Dataset Information

0

Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.

ABSTRACT: RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and reveal 'TFIIIC-only' sites. Pol III localization in other transformed and primary cell lines reveals previously uncharacterized and cell type-specific Pol III loci as well as one microRNA. Notably, only a fraction of the in silico-predicted Pol III loci are occupied. Many occupied Pol III genes reside within an annotated Pol II promoter. Outside of Pol II promoters, occupied Pol III genes overlap with enhancer-like chromatin and enhancer-binding proteins such as ETS1 and STAT1. Moreover, Pol III occupancy scales with the levels of nearby Pol II, active chromatin and CpG content. These results suggest that active chromatin gates Pol III accessibility to the genome.

SUBMITTER: Oler AJ 

PROVIDER: S-EPMC2945309 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.

Oler Andrew J AJ   Alla Ravi K RK   Roberts Douglas N DN   Wong Alexander A   Hollenhorst Peter C PC   Chandler Katherine J KJ   Cassiday Patrick A PA   Nelson Cassie A CA   Hagedorn Curt H CH   Graves Barbara J BJ   Cairns Bradley R BR  

Nature structural & molecular biology 20100425 5

RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and reveal 'TFIIIC-only' sites. Pol III localization in other transformed and primary cell lines reveals previously uncharacterized and cell type-specific Pol III loci as well as one microRNA. Notably, onl  ...[more]

Similar Datasets

Genomics Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.
2010-04-14 | E-GEOD-20309 | biostudies-arrayexpress
Genomics Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.
2010-04-14 | GSE20309 | GEO
Transcriptomics RNA Pol III transcriptomes and relationships to Pol II promoters, enhancer-binding factors and chromatin domains
2010-04-14 | E-GEOD-20609 | biostudies-arrayexpress
Genomics RNA Pol III transcriptomes and relationships to Pol II promoters, enhancer-binding factors and chromatin domains
2010-04-14 | GSE20609 | GEO
Unknown Enhancer SINEs Link Pol III to Pol II Transcription in Neurons.
| S-EPMC5732322 | biostudies-literature
Unknown Close association of RNA polymerase II and many transcription factors with Pol III genes.
| S-EPMC2840497 | biostudies-literature
Unknown Genome-wide analyses of chromatin interactions after the loss of Pol I, Pol II, and Pol III.
| S-EPMC7331254 | biostudies-literature
Unknown The Pol II preinitiation complex (PIC) influences Mediator binding but not promoter-enhancer looping.
| S-EPMC8336890 | biostudies-literature
Genomics Close Association of RNA Polymerase II and Many Transcription Factors With Pol III Genes
2009-12-31 | GSE19551 | GEO
Unknown RNA polymerase III transcriptomes in human embryonic stem cells and induced pluripotent stem cells, and relationships with pluripotency transcription factors.
| S-EPMC3896398 | biostudies-literature