Ontology highlight
ABSTRACT:
SUBMITTER: Sardar HS
PROVIDER: S-EPMC2946434 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Sardar Harjinder S HS Luczak Vincent G VG Lopez Maria M MM Lister Bradford C BC Gilbert Susan P SP
Current biology : CB 20100901 18
Centromere protein CENP-E is a dimeric kinesin (Kinesin-7 family) with critical roles in mitosis, including establishment of microtubule (MT)-chromosome linkage and movement of mono-oriented chromosomes on kinetochore microtubules for proper alignment at metaphase [1-9]. We performed studies to test the hypothesis that CENP-E promotes MT elongation at the MT plus ends. A human CENP-E construct was engineered, expressed, and purified, and it yielded the CENP-E-6His dimeric motor protein. The resu ...[more]