Ontology highlight
ABSTRACT:
SUBMITTER: Foley TL
PROVIDER: S-EPMC2948233 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Organic & biomolecular chemistry 20100820 20
In high throughput screening (HTS) campaigns, the quality and cost of commercial reagents suitable for pilot studies often create obstacles upon scale-up to a full screen. We faced such challenges in our efforts to implement an HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile route to the synthetic preparation of reactive tetraethylrhodamine and quencher probes, and their appli ...[more]