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Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA.

ABSTRACT: BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1.

SUBMITTER: Liu J

PROVIDER: S-EPMC2952495 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Human BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA.

Liu Jie J Doty Tammy T Gibson Bryan B Heyer Wolf-Dietrich WD

Nature structural & molecular biology 20100822 10

BRCA2 is a tumor suppressor that functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on single-stranded DNA (ssDNA) to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2 and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to ssDNA coated by replication protein A (RPA), in a manner that is stimulated by DSS1. ...[more]

PMID: 20729859

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