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Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.


ABSTRACT: A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans.

SUBMITTER: Tanomsing N 

PROVIDER: S-EPMC2953080 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Accurate and sensitive detection of Plasmodium species in humans by use of the dihydrofolate reductase-thymidylate synthase linker region.

Tanomsing Naowarat N   Imwong Mallika M   Theppabutr Sasikrit S   Pukrittayakamee Sasithon S   Day Nicholas P J NP   White Nicholas J NJ   Snounou Georges G  

Journal of clinical microbiology 20100811 10


A nested-PCR protocol based on the linker region of the Plasmodium dihydrofolate reductase-thymidylate synthase gene (dhfr-ts) was developed. This provides highly sensitive specific detection and identification of the five parasite species that infect humans. ...[more]

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