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Ratiometric and intensity-based zinc sensors built on rhodol and rhodamine platforms.

ABSTRACT: A xanthene-forming condensation reaction yields rhodol and rhodamine dyes carrying a zinc-binding ligand that includes the aniline-type nitrogen donor of the fluorophores. Upon zinc coordination in neutral aqueous solution, rhodol RF3 behaves as a ratiometric sensor, and rhodamine RA1 acts as a turn-off intensity-based indicator. Both fluorescent compounds bind the divalent zinc cation with micromolar affinity.

SUBMITTER: Tomat E

PROVIDER: S-EPMC2956166 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Ratiometric and intensity-based zinc sensors built on rhodol and rhodamine platforms.

Tomat Elisa E Lippard Stephen J SJ

Inorganic chemistry 20101001 20

A xanthene-forming condensation reaction yields rhodol and rhodamine dyes carrying a zinc-binding ligand that includes the aniline-type nitrogen donor of the fluorophores. Upon zinc coordination in neutral aqueous solution, rhodol RF3 behaves as a ratiometric sensor, and rhodamine RA1 acts as a turn-off intensity-based indicator. Both fluorescent compounds bind the divalent zinc cation with micromolar affinity. ...[more]

PMID: 20860360

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