Ontology highlight
ABSTRACT:
SUBMITTER: Nefedov M
PROVIDER: S-EPMC2957146 | biostudies-literature | 2011
REPOSITORIES: biostudies-literature
Nefedov Mikhail M Carbone Lucia L Field Matthew M Schein Jacquie J de Jong Pieter J PJ
Journal of biomedicine & biotechnology 20101013
We have developed a new approach to screen bacterial artificial chromosome (BAC) libraries by recombination selection. To test this method, we constructed an orangutan BAC library using an E. coli strain (DY380) with temperature inducible homologous recombination (HR) capability. We amplified one library segment, induced HR at 42°C to make it recombination proficient, and prepared electrocompetent cells for transformation with a kanamycin cassette to target sequences in the orangutan genome thro ...[more]