Prediction of protein crystallization outcome using a hybrid method.
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ABSTRACT:
SUBMITTER: Zucker FH
PROVIDER: S-EPMC2957526 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Publications
Prediction of protein crystallization outcome using a hybrid method.
Zucker Frank H FH Stewart Christine C dela Rosa Jaclyn J Kim Jessica J Zhang Li L Xiao Liren L Ross Jenni J Napuli Alberto J AJ Mueller Natascha N Castaneda Lisa J LJ Nakazawa Hewitt Stephen R SR Arakaki Tracy L TL Larson Eric T ET Subramanian Easwara E Verlinde Christophe L M J CL Fan Erkang E Buckner Frederick S FS Van Voorhis Wesley C WC Merritt Ethan A EA Hol Wim G J WG
Journal of structural biology 20100327 1
The great power of protein crystallography to reveal biological structure is often limited by the tremendous effort required to produce suitable crystals. A hybrid crystal growth predictive model is presented that combines both experimental and sequence-derived data from target proteins, including novel variables derived from physico-chemical characterization such as R(30), the ratio between a protein's DSF intensity at 30°C and at T(m). This hybrid model is shown to be more powerful than sequen ...[more]
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