Ontology highlight
ABSTRACT:
SUBMITTER: Gloor GB
PROVIDER: S-EPMC2964327 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Gloor Gregory B GB Hummelen Ruben R Macklaim Jean M JM Dickson Russell J RJ Fernandes Andrew D AD MacPhee Roderick R Reid Gregor G
PloS one 20101026 10
We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol to generate millions of overlapping reads. Combinatorial sequence tagging can be used to examine hundreds of samples with far fewer primers than is required when sequence tags are incorporated at only a single end. The number of reads generated permitted satura ...[more]