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An adaptive Expectation-Maximization algorithm with GPU implementation for electron cryomicroscopy.


ABSTRACT: Maximum-likelihood (ML) estimation has very desirable properties for reconstructing 3D volumes from noisy cryo-EM images of single macromolecular particles. Current implementations of ML estimation make use of the Expectation-Maximization (EM) algorithm or its variants. However, the EM algorithm is notoriously computation-intensive, as it involves integrals over all orientations and positions for each particle image. We present a strategy to speedup the EM algorithm using domain reduction. Domain reduction uses a coarse grid to evaluate regions in the integration domain that contribute most to the integral. The integral is evaluated with a fine grid in these regions. In the simulations reported in this paper, domain reduction gives speedups which exceed a factor of 10 in early iterations and which exceed a factor of 60 in terminal iterations.

SUBMITTER: Tagare HD 

PROVIDER: S-EPMC2967204 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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An adaptive Expectation-Maximization algorithm with GPU implementation for electron cryomicroscopy.

Tagare Hemant D HD   Barthel Andrew A   Sigworth Fred J FJ  

Journal of structural biology 20100609 3


Maximum-likelihood (ML) estimation has very desirable properties for reconstructing 3D volumes from noisy cryo-EM images of single macromolecular particles. Current implementations of ML estimation make use of the Expectation-Maximization (EM) algorithm or its variants. However, the EM algorithm is notoriously computation-intensive, as it involves integrals over all orientations and positions for each particle image. We present a strategy to speedup the EM algorithm using domain reduction. Domai  ...[more]

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