Ontology highlight
ABSTRACT:
SUBMITTER: Tagare HD
PROVIDER: S-EPMC2967204 | biostudies-literature | 2010 Sep
REPOSITORIES: biostudies-literature
Tagare Hemant D HD Barthel Andrew A Sigworth Fred J FJ
Journal of structural biology 20100609 3
Maximum-likelihood (ML) estimation has very desirable properties for reconstructing 3D volumes from noisy cryo-EM images of single macromolecular particles. Current implementations of ML estimation make use of the Expectation-Maximization (EM) algorithm or its variants. However, the EM algorithm is notoriously computation-intensive, as it involves integrals over all orientations and positions for each particle image. We present a strategy to speedup the EM algorithm using domain reduction. Domai ...[more]