Local regulation of arterial L-type calcium channels by reactive oxygen species.

ABSTRACT:

Rationale

Reactive oxygen species (ROS) are implicated in the development of cardiovascular disease, and oxidants are important signaling molecules in many cell types. Recent evidence suggests that localized subcellular compartmentalization of ROS generation is an important feature of ROS signaling. However, mechanisms that transduce localized subcellular changes in redox status to functionally relevant changes in cellular processes such as Ca(2+) influx are poorly understood.

Objective

To test the hypothesis that ROS regulate L-type Ca(2+) channel activity in cerebral arterial smooth muscle.

Methods and results

Using a total internal reflection fluorescence imaging-based approach, we found that highly localized subplasmalemmal generation of endogenous ROS preceded an

SUBMITTER: Amberg GC 

PROVIDER: S-EPMC2967383 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature