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Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.

ABSTRACT: Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

SUBMITTER: Kamei K

PROVIDER: S-EPMC2970622 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.

Kamei Ken-ichiro K Ohashi Minori M Gschweng Eric E Ho Quinn Q Suh Jane J Tang Jinghua J For Yu Zeta Tak ZT Clark Amander T AT Pyle April D AD Teitell Michael A MA Lee Ki-Bum KB Witte Owen N ON Tseng Hsian-Rong HR

Lab on a chip 20100316 9

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering ...[more]

PMID: 20390128

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