Chemically defined conditions for long-term maintenance of pancreatic progenitors derived from human induced pluripotent stem cells.

ABSTRACT: Large numbers of hormone-releasing cells, approximately 109 endocrine cells, are required to treat type I diabetes patients by cell transplantation. The SOX9-positive pancreatic epithelium proliferates extensively during the early stages of pancreatic development. SOX9-positive pancreatic epithelium is thought to be an expandable cell source of β cells for transplantation therapy. In this study, we attempted to expand pancreatic progenitors (PPs: PDX1+/SOX9+) derived from four human iPSC lines in three-dimensional (3D) culture using a chemically defined medium and examined the potential of the derived PPs to differentiate into β-like cells. PPs from four human iPSC lines were maintained and effectively proliferated in a chemically defined medium containing epidermal growth factor and R-spo

SUBMITTER: Konagaya S 

PROVIDER: S-EPMC6345937 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature