Pyrosequencing enumerates and contrasts soil microbial diversity.
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ABSTRACT:
SUBMITTER: Roesch LF
PROVIDER: S-EPMC2970868 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Publications
Pyrosequencing enumerates and contrasts soil microbial diversity.
Roesch Luiz F W LF Fulthorpe Roberta R RR Riva Alberto A Casella George G Hadwin Alison K M AK Kent Angela D AD Daroub Samira H SH Camargo Flavio A O FA Farmerie William G WG Triplett Eric W EW
The ISME journal 20070705 4
Estimates of the number of species of bacteria per gram of soil vary between 2000 and 8.3 million (Gans et al., 2005; Schloss and Handelsman, 2006). The highest estimate suggests that the number may be so large as to be impractical to test by amplification and sequencing of the highly conserved 16S rRNA gene from soil DNA (Gans et al., 2005). Here we present the use of high throughput DNA pyrosequencing and statistical inference to assess bacterial diversity in four soils across a large transect ...[more]
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