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Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP.

ABSTRACT: SPP1 is a siphophage infecting the gram-positive bacterium Bacillus subtilis. It is constituted by an icosahedric head and a long non-contractile tail formed by gene products (gp) 17-21. A group of 5 small genes (gp 22-24.1) follows in the genome those coding for the main tail components. However, the belonging of the corresponding gp to the tail or to other parts of the phage is not documented. Among these, gp22 lacks sequence identity to any known protein. We report here the gp22 structure solved by X-ray crystallography at 2.35 A resolution. We found that gp22 is a monomer in solution and possesses a significant structural similarity with lactococcal phage p2 ORF 18 N-terminal "shoulder" domain.

SUBMITTER: Veesler D

PROVIDER: S-EPMC2974835 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Crystal structure of Bacillus subtilis SPP1 phage gp22 shares fold similarity with a domain of lactococcal phage p2 RBP.

Veesler David D Blangy Stéphanie S Spinelli Silvia S Tavares Paulo P Campanacci Valérie V Cambillau Christian C

Protein science : a publication of the Protein Society 20100701 7

SPP1 is a siphophage infecting the gram-positive bacterium Bacillus subtilis. It is constituted by an icosahedric head and a long non-contractile tail formed by gene products (gp) 17-21. A group of 5 small genes (gp 22-24.1) follows in the genome those coding for the main tail components. However, the belonging of the corresponding gp to the tail or to other parts of the phage is not documented. Among these, gp22 lacks sequence identity to any known protein. We report here the gp22 structure sol ...[more]

PMID: 20506290

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2020-07-30 | PXD013186 | Pride

Bacillus subtilis operon encoding a membrane receptor for bacteriophage SPP1.

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Complete Genome Sequence of Bacillus subtilis Phage {varphi}105.

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