Unknown

Dataset Information

0

Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila.


ABSTRACT: Combining RNAi in cultured cells and analysis of mutant animals, we probed the roles of known Piwi-interacting RNA (piRNA) pathway components in the initiation and effector phases of transposon silencing. Squash associated physically with Piwi, and reductions in its expression led to modest transposon derepression without effects on piRNAs, consistent with an effector role. Alterations in Zucchini or Armitage reduced both Piwi protein and piRNAs, indicating functions in the formation of a stable Piwi RISC (RNA-induced silencing complex). Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis.

SUBMITTER: Haase AD 

PROVIDER: S-EPMC2975926 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila.

Haase Astrid D AD   Fenoglio Silvia S   Muerdter Felix F   Guzzardo Paloma M PM   Czech Benjamin B   Pappin Darryl J DJ   Chen Caifu C   Gordon Assaf A   Hannon Gregory J GJ  

Genes & development 20101021 22


Combining RNAi in cultured cells and analysis of mutant animals, we probed the roles of known Piwi-interacting RNA (piRNA) pathway components in the initiation and effector phases of transposon silencing. Squash associated physically with Piwi, and reductions in its expression led to modest transposon derepression without effects on piRNAs, consistent with an effector role. Alterations in Zucchini or Armitage reduced both Piwi protein and piRNAs, indicating functions in the formation of a stable  ...[more]

Similar Datasets

2010-10-25 | E-GEOD-24108 | biostudies-arrayexpress
2010-10-25 | GSE24108 | GEO
| S-EPMC4973292 | biostudies-literature
| S-EPMC5187580 | biostudies-literature
| S-EPMC3506263 | biostudies-literature
| S-EPMC6182186 | biostudies-literature
| S-EPMC3679447 | biostudies-literature
| S-EPMC2882632 | biostudies-literature
2010-01-04 | GSE15825 | GEO
| S-EPMC4911912 | biostudies-literature