Improved method for bacterial cell capture after flow cytometry cell sorting.
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ABSTRACT:
SUBMITTER: Guillebault D
PROVIDER: S-EPMC2976227 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Publications
Improved method for bacterial cell capture after flow cytometry cell sorting.
Guillebault D D Laghdass M M Catala P P Obernosterer I I Lebaron P P
Applied and environmental microbiology 20100903 21
Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification. ...[more]
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