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Protocol for improved resolution of plasma cell subpopulations by flow cytometry.

ABSTRACT: Plasma cells are rare cells that have been notoriously difficult to detect by flow cytometry. New advances have described B220+ CD138+ plasma cells in the bone marrow that are particularly difficult to distinguish between CD138 intermediate B220+ developing B cells. Herein we describe a novel method for detecting plasma cells in the bone marrow using a combination of CD138 and Sca-1 staining.

SUBMITTER: Wilmore JR 

PROVIDER: S-EPMC5584378 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Protocol for improved resolution of plasma cell subpopulations by flow cytometry.

Wilmore Joel R JR   Jones Derek D DD   Allman David D  

European journal of immunology 20170801 8

Plasma cells are rare cells that have been notoriously difficult to detect by flow cytometry. New advances have described B220+ CD138+ plasma cells in the bone marrow that are particularly difficult to distinguish between CD138 intermediate B220+ developing B cells. Herein we describe a novel method for detecting plasma cells in the bone marrow using a combination of CD138 and Sca-1 staining. ...[more]

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