Ontology highlight
ABSTRACT:
SUBMITTER: Behan RK
PROVIDER: S-EPMC2976817 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Biochemistry 20101021 45
The aging-associated enzyme CLK-1 is proposed to be a member of the carboxylate-bridged diiron family of proteins. To evaluate this hypothesis and characterize the protein, we expressed soluble mouse CLK-1 (MCLK1) in Escherichia coli as a heterologous host. Using Mössbauer and EPR spectroscopy, we established that MCLK1 indeed belongs to this protein family. Biochemical analyses of the in vitro activity of MCLK1 with quinone substrates revealed that NADH can serve directly as a reductant for cat ...[more]