Ontology highlight
ABSTRACT:
SUBMITTER: Mo D
PROVIDER: S-EPMC2980404 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
American journal of physiology. Renal physiology 20100811 5
Here, we compared the effects of nucleofection and lipid-based approaches to introduce siRNA duplexes on the subsequent development of membrane polarity in kidney cells. Nucleofection of Madin-Darby canine kidney (MDCK) cells, even with control siRNA duplexes, disrupted the initial surface polarity as well as the steady-state distribution of membrane proteins. Transfection using lipofectamine yielded slightly less efficient knockdown but did not disrupt membrane polarity. Polarized secretion was ...[more]