Unknown

Dataset Information

0

A structural mass spectrometry strategy for the relative quantitation of ligands on mixed monolayer-protected gold nanoparticles.


ABSTRACT: It is becoming increasingly common to use gold nanoparticles (AuNPs) protected by a heterogeneous mixture of thiolate ligands, but many ligand mixtures on AuNPs cannot be properly characterized due to the inherent limitations of commonly used spectroscopic techniques. Using ion mobility-mass spectrometry (IM-MS), we have developed a strategy that allows measurement of the relative quantity of ligands on AuNP surfaces. This strategy is used for the characterization of three samples of mixed-ligand AuNPs: tiopronin:glutathione (av diameter 2.5 nm), octanethiol:decanethiol (av diameter 3.6 nm), and tiopronin:11-mercaptoundecyl(poly ethylene glycol) (av diameter 2.5 nm). For validation purposes, the results obtained for tiopronin:glutathione AuNPs were compared to parallel measurements using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) without ion mobility separation. Relative quantitation measurements for NMR and IM-MS were in excellent agreement, with an average difference of less than 1% relative abundance. IM-MS and MS without ion mobility separation were not comparable, due to a lack of ion signals for MS. The other two mixed-ligand AuNPs provide examples of measurements that cannot be performed using NMR spectroscopy.

SUBMITTER: Harkness KM 

PROVIDER: S-EPMC2980554 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

A structural mass spectrometry strategy for the relative quantitation of ligands on mixed monolayer-protected gold nanoparticles.

Harkness Kellen M KM   Hixson Brian C BC   Fenn Larissa S LS   Turner Brian N BN   Rape Amanda C AC   Simpson Carrie A CA   Huffman Brian J BJ   Okoli Tracy C TC   McLean John A JA   Cliffel David E DE  

Analytical chemistry 20101022 22


It is becoming increasingly common to use gold nanoparticles (AuNPs) protected by a heterogeneous mixture of thiolate ligands, but many ligand mixtures on AuNPs cannot be properly characterized due to the inherent limitations of commonly used spectroscopic techniques. Using ion mobility-mass spectrometry (IM-MS), we have developed a strategy that allows measurement of the relative quantity of ligands on AuNP surfaces. This strategy is used for the characterization of three samples of mixed-ligan  ...[more]

Similar Datasets

| S-EPMC3376232 | biostudies-literature
| S-EPMC9798909 | biostudies-literature
| S-EPMC7607427 | biostudies-literature
| S-EPMC3101277 | biostudies-literature
| S-EPMC2453071 | biostudies-literature
| S-EPMC4303505 | biostudies-literature
| S-EPMC4177149 | biostudies-literature
| S-EPMC3514491 | biostudies-other
| S-EPMC2684029 | biostudies-literature
| S-EPMC4624284 | biostudies-literature