Ontology highlight
ABSTRACT:
SUBMITTER: Cescutti R
PROVIDER: S-EPMC2982761 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Cescutti Rachele R Negrini Simona S Kohzaki Masaoki M Halazonetis Thanos D TD
The EMBO journal 20100924 21
TopBP1 is a checkpoint protein that colocalizes with ATR at sites of DNA replication stress. In this study, we show that TopBP1 also colocalizes with 53BP1 at sites of DNA double-strand breaks (DSBs), but only in the G1-phase of the cell cycle. Recruitment of TopBP1 to sites of DNA replication stress was dependent on BRCT domains 1-2 and 7-8, whereas recruitment to sites of DNA DSBs was dependent on BRCT domains 1-2 and 4-5. The BRCT domains 4-5 interacted with 53BP1 and recruitment of TopBP1 to ...[more]