Bishydrazide glycoconjugates for lectin recognition and capture of bacterial pathogens.

ABSTRACT: Bishydrazides are versatile linkers for attaching glycans to substrates for lectin binding and pathogen detection schemes. The ?,?-bishydrazides of carboxymethylated hexa(ethylene glycol) (4) can be conjugated at one end to unprotected oligosaccharides, then attached onto carrier proteins, tethered onto activated carboxyl-terminated surfaces, or functionalized with a photoactive cross-linking agent for lithographic patterning. Glycoconjugates of bishydrazide 4 can also be converted into dithiocarbamates (DTCs) by treatment with CS(2) under mild conditions, for attachment onto gold substrates. The immobilized glycans serve as recognition elements for cell-surface lectins and enable the detection and capture of bacterial pathogens such as Pseudomonas aeruginosa by their adsorption onto micropatterned substrates. A detection limit of 10³ cfu/mL is demonstrated, using a recently introduced method based on optical pattern recognition.

SUBMITTER: Adak AK 

PROVIDER: S-EPMC2987539 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature