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Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.

ABSTRACT: We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.

SUBMITTER: Patel M

PROVIDER: S-EPMC3232961 | biostudies-literature |

REPOSITORIES: biostudies-literature

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