Ontology highlight
ABSTRACT:
SUBMITTER: Klein RL
PROVIDER: S-EPMC2987640 | biostudies-literature | 2008 Jan
REPOSITORIES: biostudies-literature
Klein Ronald L RL Dayton Robert D RD Tatom Jason B JB Henderson Karen M KM Henning Phillip P PP
Molecular therapy : the journal of the American Society of Gene Therapy 20071023 1
We compared adeno-associated virus (AAV) serotypes for expression levels of green fluorescent protein (GFP) in the adult rat hippocampus by biophotonic imaging. Preparations of AAV serotypes 8, 9, Rh10, and Rh43 incorporating cytomegalovirus (CMV) promoter-driven GFP were purified by a CsCl method. Neither AAV Rh10 nor AAV Rh43 produced greater levels of GFP than AAV8, which was used as a reference. For AAV9, there was an increase relative to AAV8. The CsCl-purified AAV8 displayed an astroglial ...[more]