Factors affecting the STR amplification success in poorly preserved bone samples.

ABSTRACT:

Background

Factors affecting the success of short tandem repeat (STR) amplification of poorly preserved samples are generally known, but as of yet, they have seldom been systematically assessed. Using two different maximum likelihood-based methods, the relative importance of DNA quantity, degradation and inhibition in STR genotyping was studied with DNA extracts from a set of old bone samples. First, the effects of different factors related to PCR amplification were estimated with a generalized linear mixed model. Second, error rates of allelic drop-out and drop-in were estimated on the basis of the frequency and nature of mismatches between replicates.

Results

In autosomal STR analyses, the most important factor was the DNA quantity, followed by the degradation, whereas in

SUBMITTER: Putkonen MT 

PROVIDER: S-EPMC2990737 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature