Dataset Information

Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.

ABSTRACT: Precise and dynamic measurement of intracellular metabolite levels has been hampered by difficulties in differentiating between adsorbed and imported fractions and the subcellular distribution between cytosol, endomembrane compartments and mitochondria. In the present study, genetically encoded FRET (Förster resonance energy transfer)-based sensors were deployed for dynamic measurements of free cytosolic glucose and ATP with varying external supply and in glucose-transport mutants. Moreover, by using the FRET sensors in a microfluidic platform, we were able to monitor in vivo changes of intracellular free glucose in individual yeast cells. We demonstrate the suitability of the FRET sensors for gaining physiological insight by demonstrating that free intracellular glucose and ATP levels are reduced in a hxt5? hexose-transporter mutant compared with wild-type and other hxt? strains.

SUBMITTER: Bermejo C

PROVIDER: S-EPMC2992555 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.

Bermejo Clara C Haerizadeh Farzad F Takanaga Hitomi H Chermak Diane D Frommer Wolf B WB

The Biochemical journal 20101201 2

Precise and dynamic measurement of intracellular metabolite levels has been hampered by difficulties in differentiating between adsorbed and imported fractions and the subcellular distribution between cytosol, endomembrane compartments and mitochondria. In the present study, genetically encoded FRET (Förster resonance energy transfer)-based sensors were deployed for dynamic measurements of free cytosolic glucose and ATP with varying external supply and in glucose-transport mutants. Moreover, by ...[more]

PMID: 20854260

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Dataset Information

Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.

Publications

Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.

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