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ABSTRACT:
SUBMITTER: Leggans EK
PROVIDER: S-EPMC2992964 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Leggans Erick K EK Akey David L DL Smith Janet L JL Fecik Robert A RA
Bioorganic & medicinal chemistry letters 20100525 19
A general strategy to enzymatically label acyl carrier proteins (ACPs) of polyketide synthases has been developed. Incorporation of a chloromethyl ketone or vinyl ketone moiety into polyketide chain elongation intermediate mimics allows for the synthesis of CoA adducts. These CoA adducts undergo enzymatic reaction with Sfp, a phosphopantetheinyl transferase, to afford labeled CurB carrier proteins. ...[more]