Identification of tolerated insertion sites in poliovirus non-structural proteins.
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ABSTRACT:
SUBMITTER: Teterina NL
PROVIDER: S-EPMC2993843 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Publications
Identification of tolerated insertion sites in poliovirus non-structural proteins.
Teterina Natalya L NL Lauber Chris C Jensen Kenneth S KS Levenson Eric A EA Gorbalenya Alexander E AE Ehrenfeld Ellie E
Virology 20101023 1
Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in the poliovirus polyprotein that could potentially accommodate insertion of tags, transposon-mediated insertion mutagenesis was applied to the entire nonstructural protein-coding re ...[more]
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