Ontology highlight
ABSTRACT:
SUBMITTER: Zuo X
PROVIDER: S-EPMC2994717 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Zuo Xiaolei X Xiao Yi Y Plaxco Kevin W KW
Journal of the American Chemical Society 20090501 20
We herein demonstrate a sandwich assay based on single aptamer sequences is suitable for the direct detection of small molecule targets in blood serum and other complex matrices. By splitting an aptamer into two pieces, we convert a single affinity reagent into a two-component system in which the presence of the target drives formation of a complex comprised of the target and the two halves of the aptamer. To demonstrate the utility of this approach we have used single anticocaine and anti-ATP a ...[more]