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Efficient double fragmentation ChIP-seq provides nucleotide resolution protein-DNA binding profiles.


ABSTRACT: Immunoprecipitated crosslinked protein-DNA fragments typically range in size from several hundred to several thousand base pairs, with a significant part of chromatin being much longer than the optimal length for next-generation sequencing (NGS) procedures. Because these larger fragments may be non-random and represent relevant biology that may otherwise be missed, but also because they represent a significant fraction of the immunoprecipitated material, we designed a double-fragmentation ChIP-seq procedure. After conventional crosslinking and immunoprecipitation, chromatin is de-crosslinked and sheared a second time to concentrate fragments in the optimal size range for NGS. Besides the benefits of increased chromatin yields, the procedure also eliminates a laborious size-selection step. We show that the double-fragmentation ChIP-seq approach allows for the generation of biologically relevant genome-wide protein-DNA binding profiles from sub-nanogram amounts of TCF7L2/TCF4, TBP and H3K4me3 immunoprecipitated material. Although optimized for the AB/SOLiD platform, the same approach may be applied to other platforms.

SUBMITTER: Mokry M 

PROVIDER: S-EPMC2994895 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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Efficient double fragmentation ChIP-seq provides nucleotide resolution protein-DNA binding profiles.

Mokry Michal M   Hatzis Pantelis P   de Bruijn Ewart E   Koster Jan J   Versteeg Rogier R   Schuijers Jurian J   van de Wetering Marc M   Guryev Victor V   Clevers Hans H   Cuppen Edwin E  

PloS one 20101130 11


Immunoprecipitated crosslinked protein-DNA fragments typically range in size from several hundred to several thousand base pairs, with a significant part of chromatin being much longer than the optimal length for next-generation sequencing (NGS) procedures. Because these larger fragments may be non-random and represent relevant biology that may otherwise be missed, but also because they represent a significant fraction of the immunoprecipitated material, we designed a double-fragmentation ChIP-s  ...[more]

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