Ontology highlight
ABSTRACT:
SUBMITTER: Aoki ST
PROVIDER: S-EPMC2995306 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Aoki Scott T ST Settembre Ethan C EC Trask Shane D SD Greenberg Harry B HB Harrison Stephen C SC Dormitzer Philip R PR
Science (New York, N.Y.) 20090601 5933
Rotavirus outer-layer protein VP7 is a principal target of protective antibodies. Removal of free calcium ions (Ca2+) dissociates VP7 trimers into monomers, releasing VP7 from the virion, and initiates penetration-inducing conformational changes in the other outer-layer protein, VP4. We report the crystal structure at 3.4 angstrom resolution of VP7 bound with the Fab fragment of a neutralizing monoclonal antibody. The Fab binds across the outer surface of the intersubunit contact, which contains ...[more]