Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.
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ABSTRACT:
SUBMITTER: Paskewitz SM
PROVIDER: S-EPMC2995370 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
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Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.
Paskewitz S M SM Li B B Kajla M K MK
Insect molecular biology 20080407 3
We sequenced and characterized two novel invertebrate-type lysozymes from the mosquito Anopheles gambiae. Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real-time PCR analysis. Lys i-1 transcripts were found in adult females in the fat b ...[more]
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