Dataset Information

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.

ABSTRACT:

Purpose

stem cell factor (SCF)/c-Kit regulates the proliferation and survival of germ cells or stem cells; however, little is known about the role of SCF/c-Kit in pre-implantation embryo development.

Methods

using exogenous SCF supplementation and c-Kit siRNA injection, we investigated the role and mechanism of SCF/c-Kit in pre-implantation mouse embryos.

Results

addition of soluble SCF to the culture medium improved blastocyst formation. c-Kit gene silencing reduced the rate of blastocyst formation and delayed embryonic development. The number of proliferating cells in c-Kit gene-silenced blastocysts decreased, whereas the number of apoptotic cells in blastocysts obtained from both experimental and the control groups was not affected. RT-PCR, immunostaining and western blotting revealed that proliferation-related Akt downstream targets were substantially affected by c-Kit gene silencing.

Conclusion

SCF/c-Kit signaling through Akt downstream targets is likely involved in mediating the cleavage and proliferation of blastomeres during mouse pre-implantation embryogenesis.

SUBMITTER: Lim JJ

PROVIDER: S-EPMC2995437 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Publications

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.

Lim Jung Jin JJ Eum Jin Hee JH Lee Jeoung Eun JE Kim Eun Sun ES Chung Hyung Min HM Yoon Tae Ki TK Kim Kye-Seong KS Lee Dong Ryul DR

Journal of assisted reproduction and genetics 20100630 11

<h4>Purpose</h4>stem cell factor (SCF)/c-Kit regulates the proliferation and survival of germ cells or stem cells; however, little is known about the role of SCF/c-Kit in pre-implantation embryo development.<h4>Methods</h4>using exogenous SCF supplementation and c-Kit siRNA injection, we investigated the role and mechanism of SCF/c-Kit in pre-implantation mouse embryos.<h4>Results</h4>addition of soluble SCF to the culture medium improved blastocyst formation. c-Kit gene silencing reduced the ra ...[more]

PMID: 20589425

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Dataset Information

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.

Purpose

Methods

Results

Conclusion

Publications

Stem cell factor/c-Kit signaling in in vitro cultures supports early mouse embryonic development by accelerating proliferation via a mechanism involving Akt-downstream genes.

Similar Datasets

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