Unknown

Dataset Information

0

M3-muscarinic receptor promotes insulin release via receptor phosphorylation/arrestin-dependent activation of protein kinase D1.


ABSTRACT: The activity of G protein-coupled receptors is regulated via hyper-phosphorylation following agonist stimulation. Despite the universal nature of this regulatory process, the physiological impact of receptor phosphorylation remains poorly studied. To address this question, we have generated a knock-in mouse strain that expresses a phosphorylation-deficient mutant of the M(3)-muscarinic receptor, a prototypical G(q/11)-coupled receptor. This mutant mouse strain was used here to investigate the role of M(3)-muscarinic receptor phosphorylation in the regulation of insulin secretion from pancreatic islets. Importantly, the phosphorylation deficient receptor coupled to G(q/11)-signaling pathways but was uncoupled from phosphorylation-dependent processes, such as receptor internalization and ?-arrestin recruitment. The knock-in mice showed impaired glucose tolerance and insulin secretion, indicating that M(3)-muscarinic receptors expressed on pancreatic islets regulate glucose homeostasis via receptor phosphorylation-/arrestin-dependent signaling. The mechanism centers on the activation of protein kinase D1, which operates downstream of the recruitment of ?-arrestin to the phosphorylated M(3)-muscarinic receptor. In conclusion, our findings support the unique concept that M(3)-muscarinic receptor-mediated augmentation of sustained insulin release is largely independent of G protein-coupling but involves phosphorylation-/arrestin-dependent coupling of the receptor to protein kinase D1.

SUBMITTER: Kong KC 

PROVIDER: S-EPMC3000281 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

M3-muscarinic receptor promotes insulin release via receptor phosphorylation/arrestin-dependent activation of protein kinase D1.

Kong Kok Choi KC   Butcher Adrian J AJ   McWilliams Phillip P   Jones David D   Wess Jürgen J   Hamdan Fadi F FF   Werry Tim T   Rosethorne Elizabeth M EM   Charlton Steven J SJ   Munson Sarah E SE   Cragg Hannah A HA   Smart Alison D AD   Tobin Andrew B AB  

Proceedings of the National Academy of Sciences of the United States of America 20101115 49


The activity of G protein-coupled receptors is regulated via hyper-phosphorylation following agonist stimulation. Despite the universal nature of this regulatory process, the physiological impact of receptor phosphorylation remains poorly studied. To address this question, we have generated a knock-in mouse strain that expresses a phosphorylation-deficient mutant of the M(3)-muscarinic receptor, a prototypical G(q/11)-coupled receptor. This mutant mouse strain was used here to investigate the ro  ...[more]

Similar Datasets

| S-EPMC2889095 | biostudies-literature
| S-EPMC9485632 | biostudies-literature
2022-05-06 | GSE201885 | GEO
| S-EPMC4372811 | biostudies-literature
| S-EPMC3448777 | biostudies-literature
| S-EPMC3382831 | biostudies-literature
| PRJNA833409 | ENA
| S-EPMC3059061 | biostudies-literature
| S-EPMC5514713 | biostudies-literature
| S-EPMC3292572 | biostudies-literature