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Multiplexed and reiterative fluorescence labeling via DNA circuitry.


ABSTRACT: A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed.

SUBMITTER: Duose DY 

PROVIDER: S-EPMC3002180 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Multiplexed and reiterative fluorescence labeling via DNA circuitry.

Duose Dzifa Y DY   Schweller Ryan M RM   Hittelman Walter N WN   Diehl Michael R MR  

Bioconjugate chemistry 20101116 12


A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexes to be used to label additional markers. Thus, these probes present opportunities to increase the  ...[more]

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