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NSP2 gene variation of the North American genotype of the Thai PRRSV in central Thailand.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen causing economic losses in the swine industry almost worldwide. PRRSV has been divided into 2 genotypes, the European (Type 1) and North American (Type 2) genotype, respectively and displays a large degree of genetic variability, particularly at the nonstructural protein (nsp) 2 gene. This is the first study determining genetic variation of the nsp2 of Thai PRRSV isolates. The results showed that 9 out of 10 Thai PRRSV isolates were nsp2-truncated viruses that might have evolved from a virus previously introduced in the past, but not from one recently introduced.

SUBMITTER: Kedkovid R

PROVIDER: S-EPMC3002309 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature

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NSP2 gene variation of the North American genotype of the Thai PRRSV in central Thailand.

Kedkovid Roongtham R Nuntawan Na Ayudhya Suparlark S Amonsin Alongkorn A Thanawongnuwech Roongroje R

Virology journal 20101124

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen causing economic losses in the swine industry almost worldwide. PRRSV has been divided into 2 genotypes, the European (Type 1) and North American (Type 2) genotype, respectively and displays a large degree of genetic variability, particularly at the nonstructural protein (nsp) 2 gene. This is the first study determining genetic variation of the nsp2 of Thai PRRSV isolates. The results showed that 9 out of 10 Th ...[more]

PMID: 21092327

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Project description:BackgroundGlucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common X-linked inherited erythroenzymopathy in Thailand. The clinical and hematological manifestations of G6PD deficiency are variable.ObjectiveThis study aimed to characterize the genotype-phenotype correlation of G6PD mutations in Thai pediatric patients who were followed-up in Phramongkutklao Hospital, a tertiary center in central Thailand. Material and Method. A total of 102 children including 73 males (71.6%) and 29 females (28.4%) were included in our study. Mutation analysis was performed by direct DNA sequencing of all coding exons of the G6PD gene. Ninety-one patients (89.2%) were presented with neonatal hyperbilirubinemia and 11 patients (10.8%) were presented with acute hemolytic anemia beyond the neonatal period.ResultsMolecular analysis of the G6PD gene in 102 G6PD-deficient Thai children identified 12 different mutations. G6PD Viangchan (871G?>?A) and G6PD Canton (1376G?>?T) were the first (46.2%) and the second (15.4%) most common identified mutations among both male and female G6PD-deficient individuals, respectively. All affected males were hemizygous for G6PD mutations and had an average G6PD level of 16.7?±?11.5 (3-76) IU/ml.RBC. Majority of female patients (27 in 29, 93.1%) were heterozygous for G6PD mutations and had an average G6PD level of 133.6?±?43.4 (9-195) IU/ml.RBC. Two female patients (6.9%) were either homozygous or compound heterozygous for the mutations and had G6PD level in the affected male range (35 and 10 IU/ml.RBC). Only 1 in 27 heterozygous females (3.7%) had G6PD level in the affected male range (9 IU/ml.RBC) which is possibly explained by nonrandom X-chromosome inactivation. The correlation of genotypes, G6PD levels, and clinical phenotypes was not demonstrated in our study in which all of the included G6PD-deficient patients were presented with neonatal hyperbilirubinemia and acute hemolytic anemia, since the genotype-phenotype correlation is normally demonstrated in chronic nonspherocytic hemolytic anemia (CNSHA) G6PD-deficient individuals.ConclusionThis study characterizes the molecular heterogeneity of G6PD variants causing G6PD deficiency in Thai children. Our study demonstrated the efficiency of direct DNA sequencing which can identify 12 missense mutations in Thai children.

Dataset Information

NSP2 gene variation of the North American genotype of the Thai PRRSV in central Thailand.

Publications

NSP2 gene variation of the North American genotype of the Thai PRRSV in central Thailand.

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