Activation and intrinsic gamma-secretase activity of presenilin 1.

ABSTRACT: A complex composed of presenilin (PS), nicastrin, PEN-2, and APH-1 is absolutely required for ?-secretase activity in vivo. Evidence has emerged to suggest a role for PS as the catalytic subunit of ?-secretase, but it has not been established that PS is catalytically active in the absence of associated subunits. We now report that bacterially synthesized, recombinant PS (rPS) reconstituted into liposomes exhibits ?-secretase activity. Moreover, an rPS mutant that lacks a catalytic aspartate residue neither exhibits reconstituted ?-secretase activity nor interacts with a transition-state ?-secretase inhibitor. Importantly, we demonstrate that rPS harboring mutations that cause early onset familial Alzheimer's disease (FAD) lead to elevations in the ratio of A?42 to A?40 peptides produced from a wild-type APP substrate and that rPS enhances the A?42/A?40 peptide ratio from FAD-linked mutant APP substrates, findings that are entirely consistent with the results obtained in in vivo settings. Thus, ?-secretase cleavage specificity is an inherent property of the polypeptide. Finally, we demonstrate that PEN2 is sufficient to promote the endoproteolysis of PS1 to generate the active form of ?-secretase. Thus, we conclusively establish that activated PS is catalytically competent and the bimolecular interaction of PS1 and PEN2 can convert the PS1 zymogen to an active protease.

SUBMITTER: Ahn K 

PROVIDER: S-EPMC3003001 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature