Structure analysis of group I plant nucleases.
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ABSTRACT:
SUBMITTER: Dohnalek J
PROVIDER: S-EPMC3004249 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Publications
Structure analysis of group I plant nucleases.
Dohnálek Jan J Koval' Tomáš T Lipovová Petra P Podzimek Tomáš T Matoušek Jaroslav J
Journal of synchrotron radiation 20101105 1
Anticancer drugs attacking nucleic acids of the target cells have so far been based on animal or fungal ribonucleases. Plant nucleases have been proved to exhibit decreased cytotoxic side effects. Tomato bifunctional nuclease 1 with activity against both single-stranded and double-stranded RNA and DNA was produced in tobacco leaves as recombinant protein. The enzyme crystallizes under several different crystallization conditions. The presence of Zn(2+) ions was confirmed by X-ray fluorescence. F ...[more]
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